| 发明名称 |
Expression Vector Comprising a Polynucleotide Encoding a Modified Glutamine Synthetase and a Method for Preparing a Target Protein Employing the Same |
| 摘要 |
The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant. |
| 申请公布号 |
US2015044753(A1) |
申请公布日期 |
2015.02.12 |
| 申请号 |
US201314384594 |
申请日期 |
2013.03.05 |
| 申请人 |
Hanwha Chemical Corporation |
发明人 |
Jang Hyun Sook;Lee Dong Heon;Kim Sun Kyu;Ahn Yong Ho;Park Sang Kyung |
| 分类号 |
C12N9/00 |
主分类号 |
C12N9/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A modified glutamine synthetase (GS), wherein glycine (Gly, G) at position 299 of a glutamine synthetase having an amino acid sequence shown in SEQ ID NO. 4 is substituted with arginine (Arg, R). |
| 地址 |
Seoul KR |
