Composition and method for enabling proliferation of pluripotent stem cells

摘要

The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

主权项

1. A composition that enables self-renewal of pluripotent stem cells grown in vitro, comprising a substrate including a beta fibroblast growth factor (bFGF) and only one single laminin isoform, wherein the single laminin isoform is recombinant laminin 511 (laminin-10) and the pluripotent stem cells are mouse pluripotent embryonic stem cells, human pluripotent embryonic stem cells, or human induced pluripotent stem (iPS) cells.