Isolation and purification of components of whey

摘要

A process for the isolation and/or purification of a desired component, particularly 3′sialyl lactose, from whey, particularly cheese whey, comprising the steps of: optionally subjecting the whey to ultrafiltration to provide a protein concentrate and a whey permeate; contacting the whey or whey permeate with at least one series of ion exchange resins, said series comprising a first weak acid cation resin, a first weak base anion resin, a second strong acid cation resin, a second weak base anion resin to demineralize the whey permeate; contacting this demineralized permeate with two final ion exchange resins comprising a strong acid cation resin and a final weak base anion resin, wherein the desired component, such as 3′ sialyl lactose, is taken up on the final anion resin; and eluting the desired component from the final anion resin.

主权项

1. A process for at least one of the isolation or purification of 3′-sialyl lactose, 6′-sialyl lactose, anionic oligosaccharides from whey, the process comprising the steps of:
contacting the whey with at least one group of ion exchange resins, the group comprising a first weak acid cation resin, a first weak base anion resin, a second strong acid cation resin and a second weak base anion resin to produce a demineralized whey; contacting the demineralised whey with two further ion exchange resins comprising a third strong acid cation resin followed by a weak base anion resin wherein the 3′-sialyl lactose, 6′-sialyl lactose, anionic oligosaccharides and/or proteins is taken up on the weak base anion resin; and eluting the 3′-sialyl lactose, 6′-sialyl lactose, anionic oligosaccharides and/or proteins from the weak base anion resin.