Immunoglobulin chimeric monomer-dimer hybrids

摘要

The invention relates to a chimeric monomer-dimer hybrid protein wherein the protein comprises a first and a second polypeptide chain, the first polypeptide chain comprising at least a portion of an immunoglobulin constant region and a biologically active molecule, and the second polypeptide chain comprising at least a portion of an immunoglobulin constant region without the biologically active molecule of the first chain. The invention also relates to methods of using and methods of making the chimeric monomer-dimer hybrid protein of the invention.

主权项

1. A method of making a biologically active chimeric protein comprising:
a) transfecting a cell with (i) a first DNA construct comprising a DNA molecule encoding a first polypeptide comprising a biologically active molecule fused to at least a portion of an immunoglobulin constant region and (ii) a second DNA construct comprising a DNA molecule encoding a second polypeptide comprising at least a portion of an immunoglobulin constant region without a biologically active molecule or variable region of an immunoglobulin; b) culturing the cell under conditions such that the first polypeptide encoded by said first DNA construct and the second polypeptide encoded by said second DNA construct are expressed; and c) isolating dimers comprising the first polypeptide and the second polypeptide from said transfected cell.