| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to medicine, specifically to biotechnology, and can be used for treating skin damages. Invention uses a tissue-engineered structure based on autologous fibroblast on biodegradable matrix and its application on wound. Fibroblasts transfected with, built ino plasmid vectors, genes hPOT1 (Adgene Plasmid 12387) and hPOT1 delta OB (Adgene Plasmid 13241) using a set of Lipofectamine™ 2000 (Invitrogen) according to manufacturer's protocol. Method then includes cultivation in DMEM with additionof 10 % FBS, 10 ng/ml FGF, 100 units/ml penicillin, 100 mcg/ml of streptomycin and 1 mcg/ml puramycin. During cultivation, transfected cells are selected according to resistance puramycin medium containing 1 mcg/ml puramycin.EFFECT: use of said tissue-engineered structure provides ectopic expression of telomerase genes, increases length of telomeres and high proliferative cell potential of biograft, wherein vectors function in fibroblasts of biograft for first 3-4 weeks after application, providing resistane of cells to aggressive wound content.3 cl, 11 tbl, 2 dwg |
