SAMPLE STORAGE FOR MOLECULAR AND IMMUNOLOGICAL DETECTION

摘要

A method for the direct molecular analysis of nucleic acid or antibodies from a natural or synthetic solid matrix for storing, preserving, and transporting nucleic acid or antibodies samples and simultaneously inactivating potential pathogens in the sample. A solid medium provides dry preservation of nucleic acid or antibodies, for example a filter paper treated with a novel chemical compound. This eliminates the tedious wash steps of conventional methods and allows for immediate processing of a clinical sample once it is received in the laboratory. This method is simple, rapid, and can be used to detect sensitive levels of pathogens in a complex matrix.

主权项

1. A solid medium for storing, preserving and transporting clinical or research samples of nucleic acids or antibodies, the solid medium comprising:
A natural or synthetic solid absorbent matrix; An effective amount of preservation solution to protect against degradation of nucleic acids or antibodies adsorbed onto the solid absorbent matrix, said composition comprising: A bromo-nitro-alkane-ol; A chelating agent; A buffering salt that has an ionization constant in the range pH 6-8.