| 摘要 |
A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings. |
| 主权项 |
1. A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and/or, in particular and, NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that
a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula wherein R1 represents a linear-chain or branched (C1-C4)-alkyl group or a (C1-C4)-carboxy alkyl group, is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a (C4-C8)-cycloalcanol or a compound of general formula wherein R2 and R3 are independently selected from the group consisting of H, (C1-C6) alkyl, wherein alkyl is linear-chain or branched, (C1-C6) alkenyl, wherein alkenyl is linear-chain or branched and comprises one to three double bonds, aryl, C6-C12 aryl, carboxyl, (C1-C4) carboxy alkyl, cycloalkyl, or C3-C8 cycloalkyl, is oxidized. |
