| 发明名称 |
Method for Enhanced SNP Discrimination and Detection |
| 摘要 |
The present invention describes an enhanced method for detection and discrimination of unique single nucleotide polymorphisms (SNPs) using up to 4 forced nucleotide mismatches near the 3 prime end of an allele-specific primer(s) in a PCR reaction. This method also incorporates a fluorescently labeled primer(s) for detection by capillary electrophoresis. Allele specific primers may also be tailed at the 5 prime end to enable accurate discrimination between different amplicons. |
| 申请公布号 |
US2014377745(A1) |
申请公布日期 |
2014.12.25 |
| 申请号 |
US201313921715 |
申请日期 |
2013.06.19 |
| 申请人 |
de Kloet Rolf Sybren;de Kloet Edwin;de Kloet Siwo Red;de Kloet Arne Herman;Walsh Caitlin Marie |
发明人 |
de Kloet Rolf Sybren;de Kloet Edwin;de Kloet Siwo Red;de Kloet Arne Herman;Walsh Caitlin Marie |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for genotyping and enhanced discrimination and detection of one or more unique single-nucleotide polymorphism (SNP) variants in a biological sample. |
| 地址 |
Tallahassee FL US |
