| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for inducing differentiation of a stem cell into an intestinal structure efficiently and stably without using a humoral factor.SOLUTION: A method for fabricating an intestinal structure from an embryonic stem cell and/or an artificial pluripotent stem cell includes: a process of preparing a cell culture base material including a base material, a plurality of isolated cell adhesion regions formed on the base material, and a cell non-adhesion region surrounding each cell adhesion region; a process of inoculating the embryonic stem cell and/or the artificial pluripotent stem cell on the cell culture base material; and a process of culturing the inoculated embryonic stem cell and/or artificial pluripotent stem cell in culture medium, in which the cell non-adhesion region on the cell culture base material is formed by immobilizing polyethylene glycol on the base material, the cell adhesion region is formed by oxidizing and/or decomposing at least a part of polyethylene glycol immobilized on the base material, and an area of each cell adhesion region is larger than 0.785 mm.</p> |
