发明名称 |
Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
摘要 |
The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity. |
申请公布号 |
AU2009215516(B2) |
申请公布日期 |
2014.12.18 |
申请号 |
AU20090215516 |
申请日期 |
2009.02.19 |
申请人 |
CENTOCOR ORTHO BIOTECH INC. |
发明人 |
NELSON, SHELLEY;BREVIG, THOMAS;FRYER, BENJAMIN;NIELSEN, VILLY;MARWOOD, TINA KRISTENSEN |
分类号 |
C12N5/07;C12N5/0735 |
主分类号 |
C12N5/07 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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