METHODS FOR DETERMINING TOTAL BODY SKELETAL MUSCLE MASS

摘要

The present invention is based on the finding that enrichment of D3-creatinine in a urine sample following oral administration of a single defined dose of D3-creatine can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and D3-creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass.

主权项

1. A method of determining the concentration of creatinine in a biological sample from a subject, said method comprising the steps of:
(a) obtaining a biological sample from the subject; (b) analyzing the biological sample to determine the peak area of the creatinine M+2 isotope peak for the biological sample; (c) comparing the peak area determined in step (b) to a calibration curve generated using D3-creatinine to determine the concentration of the creatinine M+2 isotope in the biological sample; (d) dividing the concentration obtained in step (c) by a dilution factor, where the dilution factor is the ratio of the concentration of creatinine M+2 to the concentration of creatinine M+0 in the biological sample.