| 摘要 |
<p>Cell reprogramming genes are loaded on a sustained expression-type Sendai virus vector free of an activity of incorporating exogenous genetic information into a chromosome. The genes are transfected into a normal differentiated cell via the vector, and expressed. Then, a vector genomic RNA including the genes is removed from the cell to establish an induced pluripotent stem cell. The Sendai virus vector makes it possible to generate an induced pluripotent stem cell having genomic information identical to that of a donor individual of the differentiated cell, in a significantly simple and efficient manner and in a safe manner with less risk of tumorigenic transformation. Thus, the Sendai virus vector can serve as an important tool for performing cell replacement therapies in a safe and efficient manner though the provision of useful induced pluripotent stem cells.</p> |
