发明名称 |
EXONUCLEASE CYCLING ASSAY |
摘要 |
The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising hybridizing the target polynucleotide with a probe to form a hybrid; exposing the hybrid to a 5′ exonuclease so that the probe in the hybrid is digested and the target polynucleotide is dissociated from the digested probe; repeating the hybridization step and the digestion step; and detecting the digested probes. The presence of the digested probes indicates the presence of the target polynucleotide. |
申请公布号 |
US2014349286(A1) |
申请公布日期 |
2014.11.27 |
申请号 |
US201414212151 |
申请日期 |
2014.03.14 |
申请人 |
GEORGETOWN UNIVERSITY |
发明人 |
Danielsen Mark;Alfonso Berenice;Tumurpurev Bolor |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method for detecting a target polynucleotide having a target sequence, comprising
(a) hybridizing the target polynucleotide with a probe having a sequence complementary with the target sequence and lacking a phosphorylated 5′ end, whereby a hybrid of the probe and the target polynucleotide is formed, wherein the hybrid comprises a hybridization region formed between the target sequence and the probe; (b) exposing the hybrid to a 5′ exonuclease, whereby the probe in the hybrid is digested and the target polynucleotide is dissociated from the digested probe; (c) repeating steps (a) and (b); and (d) detecting the digested probes; wherein the presence of the digested probes indicates the presence of the target polynucleotide. |
地址 |
Washington DC US |