AUTOLYTIC RECOMBINANT BACTERIA, PREPARATION METHOD AND APPLICATION THEREOF

摘要

The present invention discloses autolytic recombinant bacteria, a preparation method and an application thereof. The recombinant bacteria are prepared using a method comprising the following steps: introducing E7 lysis protein-encoding genes and target protein-encoding genes into starting bacteria and obtaining engineered bacteria. Said E7 lysis protein is a protein as shown in sequence 2 of the sequence table; and/or, said target protein is a quenching enzyme; specifically, said quenching enzyme can be the protein shown in sequence 5 of the sequence table. Using the method provided in the present invention to obtain recombinant bacteria having an autolytic function can reduce the recombinant bacteria wall-breaking step of the target protein preparation process, thus decreasing the production cost of the target protein.