Metal particles for surface-enhanced raman scattering and molecular sensing

摘要

A metal nanoparticle material for molecular sensing, that includes a metal nanoparticle aggregate including three to ten metal nanoparticles connected to each other through an organic molecule so that adjacent metal nanoparticles are bonded and spaced apart a predetermined distance, the aggregate containing a Raman active molecule within a field applied to the aggregate, wherein the metal nanoparticle material emits enhanced Raman scattering light from the Raman active molecule in an enhanced electric field; a method for producing the metal nanoparticle material for molecular sensing; and a molecular sensing method using the metal nanoparticle material for molecular sensing.

主权项

1. A method for producing a metal nanoparticle material for molecular sensing, the method comprising:
a) associating a single-stranded nucleic acid strand as a substrate with at least two single-stranded nucleic acid strands each having complementarity to a partial base structure in the nucleic acid strand and having one terminal with a thiol group to form a double helix, thereby obtaining a modified nucleic acid strand; b) reacting the thiol groups in the modified nucleic acid strand with a metal nanoparticle to bond the modified nucleic acid strand onto the surface of the metal nanoparticle, and then heating the resulting product at 60 to 100° C. to dissociate the double helix structure of the modified nucleic acid strand, thereby removing the single-stranded nucleic acid strand to obtain a metal nanoparticle bonded with the single-stranded nucleic acid strands through the thiol groups; c) reacting a single-stranded nucleic acid strand having complementarity to the nucleic acid strands, having one terminal with a thiol group, and having a base length equal to that of the nucleic acid strands with a metal nanoparticle, thereby obtaining a metal nanoparticle bonded with the single-stranded nucleic acid strand through the thiol group; d) mixing the metal nanoparticle bonded with the single-stranded nucleic acid strands and the metal nanoparticle bonded with the single-stranded nucleic acid strand to associate the single-stranded nucleic acid strands with the single-stranded nucleic acid strand, thereby forming a double helix to produce a metal nanoparticle aggregate; and bonding a Raman active molecule to:
the single-stranded nucleic acid strands used in a); the single-stranded nucleic acid strand used in c); or
the single-stranded nucleic acid strands used in a) and the single-stranded nucleic acid strand used in c); wherein: the metal nanoparticle material comprises a metal nanoparticle aggregate including three to ten metal nanoparticles connected to each other through an organic molecule so that adjacent metal nanoparticles are bonded and spaced apart a predetermined distance, the aggregate containing a Raman active molecule within a field applied to the aggregate; and the metal nanoparticle material emits enhanced Raman scattering light from the Raman active molecule in an enhanced electric field.