METHOD AND DEVICE FOR PRODUCING AND/OR PURIFYING POLYNUCLEOTIDES AND PRODUCTS OBTAINABLE THEREOF

摘要

An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.

主权项

1. An apparatus for obtaining a polynucleotide of interest, the apparatus is formed of circular tubes, having inlet means for introducing of media and cells or cell fractions and outlet means for collecting of contaminants and media separated from collected polynucleotide sequence of interest, said apparatus comprising:
a first pump which is connected to a vial and to a first tubing element; a mixing room; a second pump, linked to a first reservoir, said second pump having a pump output comprised between 0.1 L/min and 1 L/min and being linked to other elements of the device through said mixing room; a second tubing element starting from said mixing room having an internal diameter comprised between 0.5 cm and 5 cm, and a length comprised between 5 m to 60 m; a third pump linked to a second reservoir, said third pump being connected at the end of the second tubing element and at a third tubing element, the third pump having a pump output comprised between 0.1 L/min and 1 L/min and wherein the third tubing element has a total length comprised between 1 m and 50 m and an internal diameter comprised between 0.5 cm and 5 cm.