CDR REGIONS OF MONOCLONAL ANTIBODY THAT ANTAGONIZE SPHINOGOSINE 1-PHOSPHATE AND RELATED METHODS

摘要

Materials and method for treating cancer and screening for anti-neoplastic agents are provided. These materials and methods can include sphingosine 1-phosphate antagonists that bind to sphingosine-1 phosphate receptor subtype 3. Antibodies and aptamers that selectively bind to an epitope in the extracellular loop between transmembrane domains two and three of sphingosine-1-phosphate receptor subtype 3 are provided.

主权项

1. An isolated sphingosine-1-phosphate (S1P) antagonist that selectively binds to an epitope in the extracellular loop between transmembrane domains two and three of sphingosine-1-phosphate receptor subtype 3 (S1P3), wherein said antagonist comprises(SEQ ID NO: 2)MGFKMESQFQVFVFVFLWLSGVDGDIVMTQSHKFMSTSVGDRVSITCKASQDVSTGVVWYQQKEGQSPKLLIYSASYRYTGVPNRFTGSGSGTDFTFTISGVQAEDLAVYYCQQHYSSPTFGGGTKLEIKRADAAPTVSIFPPSSand/or(SEQ ID NO: 3)MGRLTFSFLLLIVPAYVLCQVTLKESGPGILQPSQTLSLTCSFSGFSLSTSNMGVGWIRQPSGKGLEWLLHILWNDNKHYNPALKSRLTISKDTYKNQVFLKIANVDTADTATYYCARILYRSNYYVMDYWGQGTSVTVSSAKTTPPSVYPL.