发明名称 |
EXPRESSION VECTOR AND METHOD FOR PRODUCING PROTEIN |
摘要 |
Provided are: an expression vector for secreting a protein (Z) to be recovered or a fusion protein having the protein (Z) moiety therein; a method for producing a transformant using the expression vector; the transformant; and a method for producing a protein using the transformant. An expression vector comprising an expression cassette containing a structural gene sequence (y) encoding a protein (Y), a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be recovered, and a promoter sequence and a terminator sequence for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, characterized in that the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein. |
申请公布号 |
US2014335622(A1) |
申请公布日期 |
2014.11.13 |
申请号 |
US201414337784 |
申请日期 |
2014.07.22 |
申请人 |
Asahi Glass Company, Limited |
发明人 |
KOTANI Tetsuya;Yidirei Alimujiang;Tohda Hideki |
分类号 |
C12N9/90 |
主分类号 |
C12N9/90 |
代理机构 |
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代理人 |
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主权项 |
1. An expression vector comprising an expression cassette containing a structural gene sequence (y) encoding a protein (Y), a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be recovered, and a promoter sequence and a terminator sequence for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, characterized in that the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein. |
地址 |
Tokyo JP |