| 发明名称 | Asymmetric adapter library construction | ||
| 摘要 | The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | ||
| 申请公布号 | US8883990(B2) | 申请公布日期 | 2014.11.11 |
| 申请号 | US201313794170 | 申请日期 | 2013.03.11 |
| 申请人 | New England Biolabs, Inc. | 发明人 | Mikawa Gi |
| 分类号 | C07H21/02;B01L3/00 | 主分类号 | C07H21/02 |
| 代理机构 | Bozicevic, Field & Francis, LLP | 代理人 | Keddie James S.;Bozicevic, Field & Francis, LLP |
| 主权项 | 1. A hairpin adaptor comprising: a) a double stranded stem region; and b) a loop region that comprises a cleavable linkage that is positioned between two non-complementary regions, wherein the non-complementary region that is 5′ of the cleavable linkage comprises a primer binding site that is in the range of 8 to 100 nucleotides in length. | ||
| 地址 | Ipswich MA US | ||