Method for in vitro assay of soluble fibrin by generating specific degradation products

摘要

The invention concerns a method for assaying soluble fibrin in a sample, in which said sample is brought into the presence of a plasminogen activator with a high specificity for soluble fibrin (PA-Fb sp) and the soluble fibrin count in the sample is measured by measuring the difference between the count of fibrin degradation products obtained after degrading soluble fibrin with PA-Fb sp and the base count of fibrin degradation products determined before bringing the sample into the presence of PA-Fb sp.

主权项

1. A method for assaying soluble fibrin in a sample, comprising:
measuring a difference in a count of D-dimer fibrin degradation products between a sample including Plasminogen Activator with a high specificity and/or high affinity for soluble Fibrin (PA-Fb sp) and said sample excluding said PA-Fb sp, wherein said sample which includes PA-Fb sp is incubated for minutes at 37° C. in the presence of a PA-Fb sp and said difference is then measured, wherein said PA-Fb sp is tissue plasminogen activator (t-PA) in a final concentration of about 1 μg/ml to about 2.5 μg/ml of the sample or is staphylokinase (SAK) in a final concentration of about 1 μg/ml to about 12 μg/ml of the sample.