发明名称 |
PRIMER ABLE TO REGULATE ACTIVITY BY MEANS OF DNA RESTRICTION ENZYME |
摘要 |
The present invention provides: a special primer comprising an external sequence which is not present in a target gene to be amplified, and a restriction-enzyme recognition sequence which allows the primer to be changed to a structure able to participate in a gene amplification reaction; a gene amplification method using same, which dramatically enhances specificity while also suppressing non-specific reactions, and which allows the primer concentration, amplification rate, amplification time (the time when the primer participates in the amplification reaction) and amplification extent to be regulated in accordance with the intended use. |
申请公布号 |
WO2014178562(A1) |
申请公布日期 |
2014.11.06 |
申请号 |
WO2014KR03549 |
申请日期 |
2014.04.23 |
申请人 |
GENEMATRIX INC. |
发明人 |
YOO, WANGDON;KIM, SOO-OK;HONG, SUNPYO;KIM, SUKJOON;SHIN, SOO-KYUNG;KIM, JAEIL;CHOI, HEE SOOK |
分类号 |
C12N15/11;C12Q1/68 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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