METHODS FOR IMPROVING THE DESIGN, BIOAVAILABILITY, AND EFFICACY OF RANDOM SEQUENCE POLYMER COMPOSITIONS VIA SERUM PROTEIN-BASED DETECTION OF RANDOM SEQUENCE POLYMER COMPOSITIONS

摘要

There exist in the art methods of detecting simple peptides. However, methods to determine the effective plasma concentration of mixtures of peptides as a group, rather than for individual peptides with a defined amino acid sequence, are complicated by the heterogeneity of the peptides to be detected. This application provides improved methods of detecting and assessing random sequence polymer (RSP) compositions, methods for the detection and quantitation of RSP compositions, means to determine and enrich a subset of peptides in an RSP composition based on the subset's interactions with certain capture polypeptides, and methods for administering RSP compositions to a subject in need thereof, wherein the dosage regimen and quantity may be determined or evaluated based on the above-mentioned methods for detection and quantitation.

主权项

1. A method for determining the presence of an RSP composition consisting of YEAK peptides comprising the steps:
a. affixing one or more proteins selected from the group consisting of: complement component C3; apolipoprotein A-1 preproprotein; apolipoprotein A-II preproprotein (apolipoprotein D); complement component C4A; trypsin inhibitor; inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP); alpha-1-B-glycoprotein; alpha-1-antitrypsin; apolipoprotein A-IV; ceruloplasmin; apolipoprotein E; complement factor B; prealbumin; apolipoprotein C-III; alpha2-HS glycoprotein; apolipoprotein J precursor; Coagulation factor II (thrombin); histidine-rich glycoprotein precursor; Alpha-2-HS-glycoprotein; gelsolin isoform a precursor; and inhibitor Kunitz type proteinase; to a means for quantitatively detecting said RSP composition in a sample; and b. determining the level of said RSP composition in said sample.