Antigen binding domains

摘要

A process for the production of an antigen specific antigen binding domain using a transformed host containing an expressible DNA sequence encoding the antigen specific antigen binding domain, wherein the antigen specific antigen binding domain is derived from a variable region of the immunoglobulin isotype NAR found in fish.

主权项

1. A process for the production of an antigen specific antigen binding domain comprising the steps of:
(a) immunizing a member of the Elasmobranchii subclass with a specific antigen such that the binding domain is somatically matured; (b) isolating lymphocytes from the immunizing species; (c) isolating RNA from the lymphocytes; (d) amplifying DNA sequences encoding the antigen specific antigen binding domain by PCR; (e) cloning the amplified DNA into a display vector; (f) transforming a host to produce a library; (g) selecting the desired clones from the library; (h) isolating and purifying the antigen specific antigen binding domain from these clones; (i) cloning the DNA sequences encoding the antigen specific antigen binding domain into an expression vector; (j) transforming a host with the expression vector; (k) culturing the host; and (l) isolating the antigen specific antigen binding domain so that the antigen specific antigen binding domain is produced.