| 主权项 |
1. A method for screening for a human induced pluripotent stem cell line exhibiting no differentiation resistance, comprising the steps of:
(i) measuring expression of at least one large intergenic non-coding RNA (lincRNA) or mRNA selected from group A and/or group B, (ii) selecting a human induced pluripotent stem cell line in which the lincRNA or mRNA selected from group A expresses or the lincRNA or mRNA selected from group B does not express; Group A consisting of (A1) lincRNA:chr1:852245-854050 reverse strand, (A2) GPR177, (A3) VTCN1, (A4) lincRNA:chr1:142803013-142804254 reverse strand, (A5) APOA2, (A6) WNT6, (A7) EPAS1, (A8) COL3A1, (A9) SLC40A1, (A10) S100P, (A11) HOPX, (A12) GUCY1A3, (A13) CDH10, (A14) HAPLN1, (A15) PITX1, (A16) HAND1, (A17) CGA, (A18) AQP1, (A19) DLX6, (A20) DLX5, (A21) SOX17, (A22) FLJ45983, (A23) PLCE1, (A24) H19, (A25) lincRNA:chr11:2016408-2017024 reverse strand, (A26) lincRNA:chr11:2017517-2017651 forward strand, (A27) IGF2, (A28) P2RY6, (A29) SLN, (A30) NNMT, (A31) APOA1, (A32) ERP27, (A33) LUM, (A34) CCDC92, (A35) CDX2, (A36) FLJ41170, (A37) MEG3, (A38) lincRNA:chr14:101292469-101299626 forward strand, (A39) lincRNA:chr14:101295637-101302637 forward strand, (A40) lincRNA:chr14:101296681-101298460 forward strand, (A41) lincRNA:chr14:101298129-101300147 forward strand, (A42) lincRNA:chr14:101324825-101327247 forward strand, (A43) MEG8, (A44) lincRNA:chr14:101365673-101366049 forward strand, (A45) lincRNA:chr14:101396955-101397357 forward strand, (A46) lincRNA:chr14:101430757-101433381 forward strand, (A47) lincRNA:chr14:101434059-101436282 forward strand, (A48) lincRNA:chr14:101472355-101473369 forward strand, (A49) DIO3, (A50) MEIS2, (A51) PRTG, (A52) C17orf51, (A53) lincRNA:chr17:21434064-21435857 reverse strand, (A54) lincRNA:chr17:21435180-21454915 reverse strand, (A55) lincRNA:chr17:21435959-21436405 reverse strand, (A56) CCR7, (A57) KRT23, (A58) GREB1L, (A59) GATA6, (A60) TTR, (A61) UCA1, (A62) FLRT3, (A63) lincRNA:chrX:73040495-73047819 reverse strand, (A64) VGLL1, (A65) RPS4Y1, (A66) DDX3Y, and (A67) RPS4Y2, Group B consisting of (B1) DMRTB1, (B2) lincRNA:chr1:73430887-73446112 reverse strand, (B3) lincRNA:chr1:73444697-73444997 reverse strand, (B4) C4orf51, (B5) PCDHA1, (B6) lincRNA:chr6:95250854-95263604 reverse strand, (B7) lincRNA:chr6:14280358-14285376 reverse strand, (B8) lincRNA:chr6:14283301-14285685 reverse strand, (B9) C7orf57, (B10) lincRNA:chr7:124873114-124899839 reverse strand, (B11) lincRNA:chr8:129599518-129624118 reverse strand, (B12) OC90, (B13) lincRNA:chr8:133071643-133092468 reverse strand, (B14) lincRNA:chr8:133073732-133075753 reverse strand, (B15) HHLA1, (B16) lincRNA:chr8:133076031-133093351 reverse strand, (B17) lincRNA:chr8:133090096-133097869 reverse strand, (B18) lincRNA:chr8:138387843-138421643 reverse strand, (B19) lincRNA:chr8:138418343-138425831 reverse strand, (B20) NDUFA4L2, (B21) lincRNA:chr13:54698462-54707001 reverse strand, (B22) ABHD12B, (B23) lincRNA:chr18:54721302-54731677 reverse strand, (B24) ZNF208, (B25) ZNF257, (B26) ZNF676, (B27) ZNF541, (B28) TBX1, (B29) CXorf61, and (B30) DB090170 TESTI4 Homo sapiens cDNA clone TESTI4038997 5′, mRNA sequence [DB090170]. |
