METHOD AND DEVICE FOR IDENTIFYING NUCLEOTIDE, AND METHOD AND DEVICE FOR DETERMINING NUCLEOTIDE SEQUENCE OF POLYNUCLEOTIDE

摘要

The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

主权项

1. A method of identifying a nucleotide, comprising:
a process of passing a nucleotide between electrodes a plurality of times; a process of detecting pulses of a tunnel current arising between the electrodes as the nucleotide passes through; a process of calculating a modal value of the maximum current value of each pulse; and a process of comparing the calculated modal value against a reference value.