SPATIALLY MODIFIED GENE EXPRESSION IN PLANTS

摘要

The invention provides methods of engineering plants having lignin deposition or xylan deposition that is substantially localized to the vessels of xylem tissue in the plant. The invention also provides methods of engineering plants to increase production of a desired biosynthetic product, e.g., to have increased secondary cell wall deposition or increased wax/cutin accumulation. The engineered plants of the present invention have use in bioenergy production, e.g., by improving the density and the digestibility of biomass derived from the plant and to improve water usage requirements.

主权项

1. A method of engineering a plant to increase the production of a biosynthetic product in a desired tissue, the method comprising:
introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding a transcription factor that regulates production of the biosynthetic product operably linked to a heterologous promoter, wherein the heterologous promoter is a promoter that induces gene expression of a gene that is a downstream target of the transcription factor in the desired tissue; and culturing the plant under conditions in which the transcription factor is expressed.