SUSPENSION AND CLUSTERING OF HUMAN PLURIPOTENT STEM CELLS FOR DIFFERENTIATION INTO PANCREATIC ENDOCRINE CELLS

摘要

The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.

主权项

1. A method of expanding and differentiating pluripotent cells in a dynamically agitated suspension culture system comprising culturing pluripotent cells to aggregated cell clusters in a planar adherent culture and differentiating the pluripotent cell clusters in a dynamic agitated suspension culture system, wherein the step of differentiating comprises use of a Cyp26 inhibitor.