METHOD FOR DETECTING BACILLUS ANTHRACIS

摘要

A method for detecting Bacillus Anthracis comprises steps: obtaining DNA of a sample; respectively mixing the DNA with Primer Sets pXO1, pXO2 and PL3 to form a first reactant mixture, a second reactant mixture and a third reactant mixture, wherein the Primer Sets pXO1, pXO2 and PL3 are respectively sequences specially designed for pXO1, pXO2 and PL3; respectively undertaking PCRs of the first reactant mixture, the second reactant mixture and the third reactant mixture; and detecting whether the sample contains sequences of pXO1, pXO2 and PL3 simultaneously to determine whether the sample contains Bacillus Anthracis. The present invention detects whether the sample contains pXO1, pXO2 and PL3 simultaneously to determine whether the sample contains Bacillus Anthracis and further uses specified primer sets to undertake PCRs and increase the sensitivity and specificity of detection. Thereby is increased the speed and accuracy of detection.

主权项

1. A method for detecting Bacillus Anthracis, comprising
Step S1 obtaining DNA of a sample; Step S2: respectively mixing the DNA with Primer Sets pXO1, pXO2 and PL3 to form a first reactant mixture, a second reactant mixture and a third reactant mixture, wherein a sequence of a forward primer of pXO1 is GGACACATACTAGTGAAGTACATGGAA (SEQ ID NO: 1); a sequence of a reverse primer of pXO1 is TCCTGCAGATACACTCCCACCAA (SEQ ID NO: 2); a sequence of a forward primer of pXO2 is TCTTCCCAGATAATGCATCGCT (SEQ ID NO: 3); a sequence of a reverse primer of pXO2 is CACGGAATGCTGTTTCCTCAT (SEQ ID NO: 4); a sequence of a forward primer of PL3 is CGATTGATGAAGGCGACAATGTACT (SEQ ID NO: 5); a sequence of a reverse primer of PL3 is CTCCTCGTGTGGATCGGTTGTTT (SEQ ID NO: 6); Step S3: respectively undertaking polymerase chain reactions (PCRs) of the first reactant mixture, the second reactant mixture and the third reactant mixture; and Step S4: detecting the first reactant mixture, the second reactant mixture and the third reactant mixture, and determining that the sample contains Bacillus Anthracis if the first reactant mixture, the second reactant mixture and the third reactant mixture respectively contain components of pXO1, pXO2 and PL3.