Antibody Response Phenotyping

摘要

Disclosed herein are methods, systems, mediums, and kits for use in phenotyping antibody responses via devices such as surface plasmon resonance devices. Such phentypes can include total target-specific antibody titers, quantitative isotype distribution of the target-specific antibodies, and/or epitope specificity of the target-specific antibodies. Other methods, systems, mediums, and kits are also disclosed.

主权项

1. A method for determining the phenotype of a target antigen-specific antibody response in a subject, comprising:
contacting a biorecognition surface with a sample from the subject comprising a plurality of target antigen-specific antibodies to produce a contacted surface; contacting the contacted surface with a probe antibody specific for the target antigen-specific antibodies under saturation conditions; and determining, by a biosensor device, the phenotype of the target antigen-specific antibody response in the subject.