An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec

摘要

Disclosed is a process to perform a molecular risk assessment (MRA) upon a sample suspected to contain an enterohemorrhagic Escherichia coli (EHEC), comprising the steps: a) contacting said sample or DNA isolated therefrom with a pair of primers derived from at least the following target genes: - stx1; - stx2; and at least one of the following target genes: - eae; - espK; and with a pair of primers derived from at least one of the following target genes: - nleB; - nleH1-2; - nleE; - ent/espL2; and detecting the presence or the absence of an amplification product for each of said target genes; and if the amplification products for each of said genes from step a) are detected then: b) contacting said sample or DNA isolated therefrom with one or more pairs of primers derived from the eae target gene and determining the eae subtype.