发明名称 |
Hybridoma cell line 10G4 and a monoclonal antibody against the total of aflatoxin B1, B2, G1 and G2 |
摘要 |
Hybridoma cell line 10G4 and monoclonal antibody against total aflatoxins produced by the hybridoma cell line 10G4. The hybridoma cell line 10G4 is used to produce the monoclonal antibody that binds specifically total aflatoxin B1, B2, G1 and G2. The titer of the mouse ascites antibody produced by the 10G4 treated mouse is determined through non-competitive enzyme-linked immunosorbent assay and the titer can reach up to 5.12×105. The monoclonal antibody against total aflatoxin B1, B2, G1 and G2 are used for better identification of aflatoxin B1, B2, G1 and G2 with good consistency. The 50% inhibitory concentrations (IC50) of the antibody against aflatoxin B1, B2, G1 and G2 are 2.09 ng/mL, 2.23 ng/mL, 2.19 ng/mL and 3.21 ng/mL respectively. The range of cross reaction rate for aflatoxin B1, B2, G1 and G2 is about 65.2%-100%. The antibody is used for quantitative measurement of total aflatoxin B1, B2, G1 and G2. |
申请公布号 |
US8841419(B2) |
申请公布日期 |
2014.09.23 |
申请号 |
US201313984231 |
申请日期 |
2013.01.17 |
申请人 |
Oilcrops Research Institute of Chinese Academy of Agriculture Sciences |
发明人 |
Li Peiwu;Li Xin;Zhang Qi;Ding Xiaoxia;Zhang Wen;Li Ran;Zhang Zhaowei |
分类号 |
C07K16/00;C07K16/14;G01N33/577 |
主分类号 |
C07K16/00 |
代理机构 |
Morris, Manning & Martin, LLP |
代理人 |
Xia, Esq. Tim Tingkang;Morris, Manning & Martin, LLP |
主权项 |
1. A hybridoma cell line 10G4 deposited at the China Center for Type Culture Collection (CCTCC) with the CCTCC accession number of C201016. |
地址 |
Wuhan, Hubei CN |