SPECTROSCOPY DEVICE, SPECTROSCOPY METHOD, AND COMPUTER PROGRAM FOR SPECTROSCOPY, EMPLOYING INDIVIDUAL LIGHT-EMITTING PARTICLE DETECTION

摘要

There is provided a way of enabling the discrimination or identification of the kind of a light-emitting particle corresponding to each pulse form signal in the scanning molecule counting method using the optical measurement by the confocal microscope or a multiphoton microscope. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, there are performed moving the position of a light detection region in a sample solution periodically along a predetermined route; measuring the light intensity from the light detection region to generate light intensity data; and detecting individually a signal of light from a light-emitting particle in the light intensity data. Then, an index value indicating a translational diffusional characteristic of one light-emitting particle in a plane perpendicular to the moving direction of the light detection region is determined based upon an intensity value of a signal of light of the one light-emitting particle and (an) intensity value(s) within (a) time region(s) separated from the generation time of the signal of light of the one light-emitting particle by the time corresponding to an integral multiple of a moving cycle of the position of the light detection region in the time series light intensity data, and it becomes possible to identify a light-emitting particle with the index value.