METHODS FOR ACCELERATED SELECTION OF POLYPEPTIDES

摘要

In certain embodiments, the disclosure provides a method for generating an mRNA-protein fusion molecule. In other embodiments, the disclosure provides a method for selecting a desired polypeptide.

主权项

1. A method for generating an mRNA-protein fusion molecule, comprising:
(a) contacting an in vitro transcription translation (IVTT) system with (1) a DNA molecule which comprises a protein coding sequence and a 5′ untranslated region (5′ UTR); and (2) a linker molecule which comprises (i) a nucleic acid portion which hybridizes to the 3′ end of the mRNA encoded by the DNA molecule; (ii) a peptide acceptor; and (iii) a psoralen moiety; (b) transcribing the DNA molecule into an mRNA and translating the mRNA into a protein in the IVTT system, wherein the protein is fused to the mRNA through the linker molecule; and (c) cross-linking the mRNA-protein fusion through the psoralen moiety.