Method and apparatus for cutting and collecting dissected specimens

摘要

Methods and devices for cutting and collecting dissected specimens are described herein. In one embodiment, the method for dissecting and collecting specimens includes the following steps: placing a sample onto a slide of a laser dissection microscope, the slide being translatable in its plane of extension, lowering an adherent collection device centered on the optical axis onto the probe, wherein the adherent collection device, in its lowered state, adheres to the sample, is freely translatable with the slide and decoupled from the optical axis of the microscope, and, in its raised state, is fixed relative to the optical axis, dissecting one or more specimens from the sample, wherein the following steps are performed if the next specimen to be dissected is located outside of a predetermined collection radius of the adherent collection device: raising the adherent collection device, translating the slide to a given position relative to the optical axis of the microscope in order to decenter the center of the specimen to be dissected from the optical axis of the microscope, lowering the adherent collection device onto the sample, and dissecting one or more subsequent specimens, wherein, after each step of raising the adherent collection device, the predetermined position is spaced apart from all previously determined positions by at least one predetermined distance.

主权项

1. A method for dissecting and collecting specimens, comprising:
(a) placing a sample onto a slide of a laser dissection microscope, the slide being translatable in its plane of extension, (b) cutting one or more specimens from the sample wherein each specimen is smaller than the entire sample, wherein the position of each specimen is input into a controller, and (c) collecting the dissected specimens by lowering an adherent collection device centered on the optical axis onto the sample, wherein the adherent collection device, in its lowered state, adheres to the sample, is freely translatable with the slide and decoupled from the optical axis of the microscope, and, in its raised state, is fixed relative to the optical axis, (d) wherein the following steps are performed if the next specimen to be dissected is located outside of a predetermined collection radius of the adherent collection device: (i) raising the adherent collection device, (ii) translating the slide to a given position relative to the optical axis of the microscope, in order to decenter the center of the next specimen to be dissected from the optical axis of the microscope, (iii) lowering the adherent collecting device onto the sample, (iv) adhering one or more subsequent specimens onto the adherent collection device, and (v) dissecting the one or more subsequent specimens, wherein, after each step of raising the adherent collection device, the predetermined position is spaced apart from all previously determined positions by at least one predetermined distance to allow collection of more than one specimen in a referenceable manner on a single adherent collection device, wherein, prior to each dissection, the next specimen to be dissected is centered in the optical axis of the microscope.