Method of labeling sugar chain

摘要

The method of labeling a sugar chain from a biological sample employs a single reaction vessel for the sequential performance of the following steps of (a) isolating a sugar chain from a sample using a sugar-trapping substance; (b) washing the sugar-trapping substance having the sugar chains trapped thereon; (c) releasing the sugar chain from the sugar-trapping substance; and (d) labeling the released sugar chain with UV/visible or fluorescent compound having an amino group forming a stable labeled sugar in fewer steps than a conventional ion exchange based technique.

主权项

1. A method of labeling a sugar chain, used for labeling a sugar chain contained in a biological sample, comprising:
(a) trapping a sugar chain in a sugar-trapping substance, which is a substance for specifically trapping a sugar chain from a biological sample; (c) releasing the sugar chain from said sugar-trapping substance; and (d) labeling the released sugar chain, wherein the processes (a), (c) and (d) are conducted sequentially in a single reaction vessel and in said processes (d), the sugar chain is labeled with a compound having amino group and the labeling of the sugar chain with said compound is carried out by reductive amination.