Methods and reagents for identifying/isolating T regulatory (Treg) cells and for treating individuals

摘要

An affinity ligand is reactive to the GARP protein may be capable of binding to an extracellular domain of GARP protein expressed on regulatory T (Treg) cells. The affinity ligand may be an antibody and may be used to identify Treg cells. A method comprises providing a blood sample from a subject and determining the amount of Treg cells in that sample. A composition containing Treg cells may be administered to an individual to suppress effector T cell activity in the individual. A composition containing an affinity ligand capable of binding to a GARP domain may be administered to an individual to suppress Treg cell activity and increase effector T cell activity in the individual. A kit for detecting Treg cells may include an affinity ligand reactive with mammalian GARP protein.

主权项

1. A method for screening cells for identity as regulatory T cells, the method comprising:
providing a blood sample from a subject; and determining the amount of regulatory T cells in a specified volume of blood by identifying T cells in at least a portion of the blood sample that express glycoprotein A repetitions predominant (GARP) protein on the surface of the cells; wherein the cells expressing a GARP protein on the surface are identified by reacting with an antibody raised against at least a portion of the GARP protein.