Disk diffusion assay for oritavancin

摘要

The present invention is directed to a disk diffusion assay for determining susceptibility of bacteria to a glycopeptide antibiotic. The assay includes improvements over conventional assays due to the inclusion of polysorbate 80 and Span 80 in the antibacterial solution used to impregnate paper disks used in the assay.

主权项

1. A disk diffusion assay for determining susceptibility of bacteria to oritavancin, said assay comprising:
a) impregnating a paper disk with a solution comprising oritavancin, polysorbate 80 and sorbitane monooleate (Span 80), b) placing the impregnated disk on the surface of a bacteria-coated media plate, c) incubating the plate of b) under conditions promoting bacterial growth, and d) measuring a zone of inhibition around the disk.