发明名称 Blood analyzer, blood analysis method and hemolytic agent
摘要 This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information.
申请公布号 US8808624(B2) 申请公布日期 2014.08.19
申请号 US201012942561 申请日期 2010.11.09
申请人 Sysmex Corporation 发明人 Matsumoto Hideaki;Uchihashi Kinya;Itose Yuji;Konishi Aya
分类号 G01N33/00 主分类号 G01N33/00
代理机构 Sughrue Mion, PLLC 代理人 Sughrue Mion, PLLC
主权项 1. A blood analyzer comprising: a sample preparation portion configured to prepare a first measurement sample containing a blood sample and a hemolytic agent and a second measurement sample containing said blood sample and the same hemolytic agent as said hemolytic agent, wherein a dilution magnification of said hemolytic agent in said second measurement sample is different from a dilution magnification of said hemolytic agent in said first measurement sample; a first measurement portion including a flow cell configured to connect with the sample preparation portion, a light source that irradiates light to the flow cell, and first, second and third light detectors, wherein the first, second and third light detectors respectively receive a first type of scattered light, a second type of scattered light, and fluorescence emitted from the flow cell and the light detectors output signals according to intensities of the received light; a second measurement portion including a cell configured to connect with the sample preparation portion and a light source that irradiates light to the cell and generating either transmitted light information or scattered light information according to transmitted light or scattered light from the cell; and a data processing unit is connected to the first and second measurement portions via a communication interface and comprises a memory storing a computer program, wherein the data processing unit is configured to execute the computer program from the memory, wherein the computer program is programmed to perform a first analysis operation and a second analysis operation steps, wherein; (A) the data processing unit is configured to perform the first analysis operation, the first analysis operation includes steps of: receiving signals from the first measurement portion via the communication interface; determining a proportion of monocyte, granulocyte and a group of lymphocyte and basophil contained in the first measurement sample based on the signals output from the first and second light detectors; determining a proportion of eosinophil in the first measurement sample based on the signals output from the first and third light detectors; and calculating a proportion of monocyte, neutrophil, and eosinophil in the first measurement sample based on the determination results, and (B) the data processing unit is configured to perform the second analysis operation, the second analysis operation includes steps of: receiving transmitted light information or scattered light information generated by the second measurement portion via the communication interface; and acquiring a hemoglobin concentration of the second measurement sample on the basis of the received information from the second measurement portion.
地址 Kobe JP