Crystal structure of the N-terminal domain of angiotensin converting enzyme and uses thereof

摘要

The present invention relates to a crystal. In particular the present invention relates to a crystal of the N-domain of ACE protein. The present invention also relates to methods, processes, domain specific modulators, pharmaceutical compositions and uses of the N-domain crystal and the structure co-ordinates thereof.

主权项

1. A method of preparing a crystal of the N-domain of ACE protein consisting of an amino acid sequence defined by amino acids 1-629 of SEQ ID NO: 8, the method comprising the steps of:
(a) culturing host cells that express the N-domain of ACE protein; (b) purifying the N-domain of ACE protein; and (c) crystallising the N-domain of ACE protein using about 5 mM HEPES and about 0.1 mM phenylmethylsulfonylfluoride (PMSF) with an equal volume of a reservoir solution containing about 0.2M lithium sulphate, 15% PEG 4000, about 100 mM CH3COONa.3H2O pH 4.9 and about 10 μM ZnSO4.7H2O.