| 摘要 |
The present invention relates to a method for preparing D-chiro-inositol by reacting myo-inositol, which is a substrate, in an enzymatic reaction buffer solution containing inositol dehydrogenase and inosose isomerase, or a fusion protein of the enzymes. D-chiro-inositol is produced in an extracellular enzymatic reaction buffer solution by the method of the present invention, and thus it is possible to produce D-chiro-inositol at low cost and in a high yield compared with a fermentation method using a recombinant cell. In addition, it is possible to separate D-chiro-inositol in a high purity from a mixture reaction buffer solution containing myo-inositol and D-chiro-inositol by a simple process such as the addition of an organic solvent, the separation of a supernatant and drying. Additionally, myo-inositol, which is separated from D-chiro-inositol by the present invention, can be reused as a substrate. |
