发明名称 |
RT-QPCR ANALYSIS OF MICRO-DISSECTED MATERIAL FROM STAINED FFPET SECTION |
摘要 |
The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70°C for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method. |
申请公布号 |
WO2014114650(A1) |
申请公布日期 |
2014.07.31 |
申请号 |
WO2014EP51157 |
申请日期 |
2014.01.22 |
申请人 |
ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG;ROCHE MOLECULAR SYSTEMS, INC. |
发明人 |
BAHLE, BEATRIX;HEROLD, ANDREA;LOHMANN, SABINE;MOOSMANN, SABINE;SCHUSTER, JULIAN;SINGER, MONIKA |
分类号 |
G01N1/30 |
主分类号 |
G01N1/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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