HIGH THROUGHPUT TRANSCRIPTOME ANALYSIS

摘要

Kits and methods for single cell or multiple cell transcriptome analysis are provided. An adapter polynucleotide is disclosed which comprises a double- stranded DNA portion of 15 base pairs and no more than 100 base pairs with a 3'single stranded overhang of at least 3 bases and no more than 10 bases, wherein said double stranded DNA portion is at the 5' end of the polynucleotide and wherein the sequence of said 3'single stranded overhang is selected from the group consisting of SEQ ID NOs: 1-8 and 9, wherein the 5' end of the strand of said double-stranded DNA which is devoid of said 3'single stranded overhang comprises a free phosphate.