D-PSICOSE 3-EPIMERASE MUTANT WITH IMPROVED THERMAL STABILITY, AND CONTINUOUS PRODUCTION OF D-PSICOSE USING SAME

摘要

The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.

主权项

1. A D-psicose 3-epimerase variant with improved thermostability, wherein the variant is a variant of a wild type D-psicose 3-epimerase derived from Agrobacterium tumefaciens and has an amino acid sequence in which isoleucine (Ile) at position 33 of the amino acid sequence is substituted with an amino acid selected from the group consisting of leucine (Leu), cysteine (Cys) and valine (Val), or an amino acid sequence in which serine (Ser) at position 213 of the amino acid sequence is substituted with cysteine.