发明名称 |
LONG RIGID SPACERS TO ENHANCE BINDING KINETICS IN IMMUNOASSAYS |
摘要 |
<p>The present invention relates to a device for detecting a target molecule within a sample comprising a sample container for the measurement of the target molecule within a sample, a first particle,wherein said first particle is functionalized with a first binding molecule capable of specifically binding to said target molecule, and a surface structure comprising a second binding molecule, wherein said surface structure covers a flat sensor or is present on a second particle, wherein said first particle is capable of binding said second binding molecule of the surface structure directly or indirectly; wherein said first and/or second binding molecule is indirectly attached to the particle surface of said first and/or second particle and/or the flat sensor surface via a long and rigid linker molecule; wherein the length and the consistency of said linker molecule is selected such as to result in an average extension length of said linker of more than 60 nm; and wherein the number of particle clusters or of bound particles is directly or inversely related to the amount of target molecules present in the sample.In a further aspect the present invention relates to a method of detecting the presence or amount of a target molecule within a sample. The present invention also describes the use of a particle according to the invention for detecting a target molecule within a sample.</p> |
申请公布号 |
EP2745091(A1) |
申请公布日期 |
2014.06.25 |
申请号 |
EP20120799638 |
申请日期 |
2012.11.13 |
申请人 |
KONINKLIJKE PHILIPS N.V. |
发明人 |
EVERS, TOON, HENDRIK;KOETS, MAATJE |
分类号 |
G01N21/00;G01N33/52;G01N33/543;G01N33/553;G01N33/58 |
主分类号 |
G01N21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|