| 主权项 |
1. A method for determining the concentration values of key constituent chemicals of honey, comprising the steps of:
(a) estimating the botanical origin of at least one standard honey sample, by:
(i) obtaining key constituent chemical concentrations; and(ii) assigning the botanical origin as a numerical value on the basis of abundance of chemical compounds characteristic of certain botanical groups, wherein the numerical value is capable of reflecting an estimation that the standard honey sample includes honey from more than one botanical origin; (b) generating the fluorescence signature of standard honey samples, by:
(i) exciting a diluted honey sample solution with light of wavelengths over the range 200-700 nm at increasing wavelength increments; and(ii) measuring the intensity of the fluorescent light emitted from the excited solution over the range 280-650 nm at increasing wavelength increments; and(iii) measuring the wavelength of the fluorescent light emitted from the excited solution over the range 280-650 nm at increasing wavelength increments; and(iv) combining excitation and emitted light as 2-dimensional excitation-emission matrix (EEM); (c) constructing a validated predictive mathematical model from standard honey data, by:
(i) using the botanical origin value determined in step (a) as the first matrix in a multivariate analysis;(ii) using the fluorescence EEM data determined in step (b) as the second matrix in a multivariate analysis;(iii) generating a mathematical model using these two matrices; and,(iv) establishing a statistical confidence of predictive power of mathematical model with leave-one-out validation process; (d) generating the fluorescence EEM signature of an unknown honey sample or samples, as outlined in step (b); and (e) using the unknown honey fluorescence EEM data from step (d) with the validated mathematical model of step (c) to predict and assign concentration values of key constituent chemicals of honey with defined statistical confidence.
|
