Surface immobilised multilayer structure of vesicles

摘要

A surface-immobilized multilayer structure of a plurality of vesicles (2), the structure comprising at least one linker (4) immobilized onto the surface, the at least one linker (4) being bound to at least one other linker, which is attached to a vesicle, which optionally may have another linker (5) bound to another linker (5) attached to another vesicle (2), wherein the structure either comprises at least two vesicles (2) bound via linkers (5) to each other or at least two vesicles (2) bound via linkers (5) attached to the vesicles to one linker (4) immobilized onto the surface (1).

主权项

1. A biologically-functional, surface-immobilized multilayer structure, comprising (i) a support surface, (ii) a plurality of first vesicles formed of a lipid bilayer membrane and spaced apart from said surface, and (iii) a plurality of second vesicles formed of a lipid bilayer membrane and spaced apart from said surface,
wherein the plurality of first vesicles is directly attached to the surface by binding surface-immobilized linkers with vesicle-attached linkers to form a first immobilized layer of the plurality of first vesicles, wherein the plurality of the second vesicles is attached to the first immobilized layer of the plurality of first vesicles by binding vesicle-attached linkers to vesicle-attached linkers to form two or more immobilized layers of the plurality of second vesicles, wherein the surface-immobilized linkers and the vesicle-attached linkers comprise oligonucleotides and binding of one linker to another linker is mediated through hybridization of said linker oligonucleotides; and wherein at least a selected population of said vesicles comprise a biologically active compound which provides the structure with biological functionality.