| 摘要 |
Background: Autosomal dominant polycystic kidney disease (ADPKD) shows a characteristic that kidney function is lost by abnormal proliferation of kidney epithelial cells. Even with the confirmation of gene which causes the ADPKD, effective medicine for the disease has not found so far. The functions of multidrug-resistance associated protein 3 (MRP3) in kidney are merely known. Method: RNAi system and Abcc3 clone for restoration are used to check the functions of the MRP3 in kidney. Fluorescent immunohistochemistry is performed to estimate expression of MRP3 in vivo. Result: MRP3 inhibition by MK571 or siRNA stimulates cell proliferation through ERK/B-Raf signaling pathway. In addition, expression of MRP3 in vivo is confirmed. MRP3 in kidneys of ADPKD patients is down regulated. However, restoration of the MRP3 reduces proliferation and cystogenesis in vitro. Conclusion: Such result tells that the functions of the MRP3 in kidney are related to cell proliferation, and restoration of the MRP3 is effective in polycystic kidney disease treatment. |
