| 摘要 |
A method for attenuation of Marek disease virus of the first serotype comprises cells cultivation, infection of cell culture with infecting dose, overseeing of cytopathic changes in the cell cultures (virus replication in cell cultures), gathering from culture dish of virus-containing biomass. The infection dose 1000TCD/ml is used, the virus-containing biomass is gathered from culture dish after 4 days, and primary trypsin cell culture and passaged cell cultures are used a cell systems. |
