发明名称 |
Preparation of templates for nucleic acid sequencing |
摘要 |
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction. |
申请公布号 |
US8715966(B2) |
申请公布日期 |
2014.05.06 |
申请号 |
US201213450920 |
申请日期 |
2012.04.19 |
申请人 |
XIAOHAI LIU;MILTON JOHN;SMITH GEOFFREY PAUL;BARNES COLIN LLOYD;RASOLONJATOVO ISABELLE MARIE JULIA;RIGATTI ROBERTO;WU XIAOLIN;OST TOBIAS WILLIAM BARR;WORSLEY GRAHAM JOHN;EARNSHAW DAVID JAMES;TURCATTI GERARDO;ROMIEU ANTHONY;ILLUMINA CAMBRIDGE LIMITED |
发明人 |
XIAOHAI LIU;MILTON JOHN;SMITH GEOFFREY PAUL;BARNES COLIN LLOYD;RASOLONJATOVO ISABELLE MARIE JULIA;RIGATTI ROBERTO;WU XIAOLIN;OST TOBIAS WILLIAM BARR;WORSLEY GRAHAM JOHN;EARNSHAW DAVID JAMES;TURCATTI GERARDO;ROMIEU ANTHONY |
分类号 |
C12Q1/68;C12P19/34;C40B40/06 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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