发明名称 Method for detecting compounds modulating dimers of VFT domain membrane proteins
摘要 The invention relates to a method for selecting compounds having a modulating effect on the activation state of a dimer of VFT-domain proteins expressed in cell membranes present in a measuring medium, said dimer consisting of a first protein and of a second protein, said proteins being identical or different, wherein this method comprises the following steps: (a) labeling the first and second proteins in the N-terminal portion of their VFT domains with the members of a pair of FRET partners, the Förster radius (R0) of said pair being between 20 and 55Å; (b) measuring the FRET signal in the absence and in the presence of the test compound within a predetermined time window; (c) selecting the test compound as a modulating compound if a difference in FRET signal in the absence and in the presence of test compound is measured in step (b). The invention can be used in the search for new medicaments and new taste modulators.
申请公布号 US8697380(B2) 申请公布日期 2014.04.15
申请号 US201013266222 申请日期 2010.04.29
申请人 DOUMAZANE ETIENNE;ZWIER JURRIAAN;TRINQUET ERIC;PIN JEAN-PHILLIPPE;CIS BIO INTERNATIONAL;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE 发明人 DOUMAZANE ETIENNE;ZWIER JURRIAAN;TRINQUET ERIC;PIN JEAN-PHILLIPPE
分类号 C12Q1/02;C12Q1/34;C12Q1/48 主分类号 C12Q1/02
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